Figure 2.

Dose-dependent effects of baclofen on (A) the synthesis of inositol phosphate (shown as percentages of basal concentrations) and (B) the peak (transient) increase in intracellular Ca²⁺ (shown as change in fluorescence [ΔF] from baseline fluorescence [Fo]) in human airway smooth muscle cells. *P < 0.05 and **P < 0.01, compared with basal concentrations. The dose-dependent effects of baclofen on (C) the bradykinin-stimulated synthesis of inositol phosphate and (D) the peak intracellular increase of Ca²⁺ in human airway smooth muscle cells are shown as a percentage of the response to 1 μM bradykinin. *P < 0.05, **P < 0.01, and ***P < 0.001, compared with bradykinin. Data represent means ± SEM. Numbers of experiments are shown in parentheses.