Figure 3.

Transcription of PcPET8 by P. carinii. A “one-step” RT-PCR reaction was performed using total RNA isolated from P. carinii and primers, based on the PcPET8 sequence. Lane 1, DNase-treated total RNA template. The product demonstrates the presence of PcPET8 transcript in RNA. Lane 2, DNase-treated total RNA template, with omission of reverse transcriptase. The lack of product demonstrates a lack of genomic DNA contamination in the DNase-treated RNA template. Lane 3, Genomic DNA template, with omission of reverse transcriptase. The correct size of the product shows primer specificity, and confirms the lack of introns.