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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Dev Biol. 2011 Nov 6;362(1):24–41. doi: 10.1016/j.ydbio.2011.10.033

Figure 10.

Figure 10

Epithelial HIF1a TPM expression induces lymphangiogenesis in the embryonic lung. A) qPCR analysis of lymphangiogenic markers in HIF1a TPM lungs on dox from E6.5 until harvest at E14.5. n=3–5 samples per genotype. *p<0.05 vs. CON. B–E) PROX-1 immunostaining in E14.5 lungs. Note increased numbers of PROX1+ endothelial cells in lymphatic vessels of HIF1a TPM lungs (C vs. B, arrows) and PROX1+ staining in neuroendocrine cells of the epithelium (B and C, arrowheads). An increase in the number and size of PROX1+/FLT4+ lymphatic vessels was also observed in HIF1a TPM lungs compared to CON (panels D and E, arrowheads). Scale bars = 100μm. F) qPCR analyses demonstrates increased expression of the Notch signaling pathway genes in HIF1a TPM lungs. n=3–6 samples per genotype. *p<0.05 vs. CON. G) Notch1 signaling is increased in the mesenchyme subtending the proximal epithelium of HIF1a TPM lungs (arrows), as demonstrated by immunofluorescent staining with the cleaved Notch1 antibody Val1744. Scale bars = 200μm.