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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Dev Biol. 2011 Nov 6;362(1):24–41. doi: 10.1016/j.ydbio.2011.10.033

Figure 5.

Figure 5

Decreased maturation of distal epithelial cells in HIF1a TPM lungs at E18.5. A–D) Note abundant surfactant in airspaces of CON lungs (arrows, panels A and C) compared to absence of surfactant in HIF1a TPM lungs (panels B and D) in toluidine-stained sections and electron micrographs. Alveolar type II cells from CON lungs contained lamellar bodies (arrowheads, panel C) while the distal epithelium HIF1a TPM lungs were devoid of lamellar bodies and contained abundant glycogen stores (panel D vs. C). G = glycogen, N = nucleus. Scale bar = 30μm for panels A and B, 2μm for panels C and D. E) Glycogen assays demonstrate increased glycogen stores in HIF1a TPM lungs at E18.5 vs. CON. Total glycogen content was normalized to genomic DNA. n=6 samples per genotype. *p<0.05 vs. CON. F) Saturated phosphatidylcholine (SatPC) is significantly decreased in E18.5 HIF1a TPM lungs vs. CON. n=5 samples per genotype. *p<0.05 vs. CON.