Figure 3.

Acetylation status of histones is important for cell-specific expression of EC-SOD. (A) HPAECs and HPASMCs were exposed to 5-aza-2′-deoxycytidine (5-aza-dC; 0.5 μM and 2 μM) or trichostatin A (TSA; 1.5 μM and 3 μM), or a combination of both substances for 48 hours. (B) Cells were exposed to DMSO or 2 μM of 5-aza-dC for 96 hours. Total RNA was extracted, and EC-SOD mRNA was measured by quantitative real-time RT-PCR. The data were normalized to concentrations of GAPDH mRNA. The results shown represent the mean ± SD from at least two independent experiments. *P < 0.01, significant difference, according to ANOVA/Bonferroni post hoc test.