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Regulation of EC-SOD and gp91phox transcription by cytokines. MRC5 cells (A and B) and HPASMCs (C–F) were exposed to 5,000 U/ml of IFN-γ (A, C, and E) or 30 ng/ml of TNF-α (B, D, and F) for the times indicated. Total RNA was extracted, and EC-SOD and gp91phox mRNA was measured by quantitative real-time RT-PCR. The data were normalized to concentrations of GAPDH mRNA. The results shown represent the mean ± SD from at least two independent experiments.
