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. 2011 Nov;45(5):953–961. doi: 10.1165/rcmb.2011-0012OC

Figure 5.

Figure 5.

Role of Janus tyrosine kinase (JAK)/signal transducers and activators of transcription proteins (STAT) signaling pathway in IFN-γ–dependent induction of EC-SOD. (A) Time course of inducible expression of interferon-responsive factor–1 (IRF-1) and EC-SOD in HPASMCs. Cells were incubated with 5,000 U/ml of IFN-γ for the indicated times. (B and C) Effects of JAK1, JAK2 (AG490), and STAT3 inhibitors on IFN-γ–induced EC-SOD (B) and IRF-1 (C) mRNA concentrations in HPASMCs. Cells were incubated with vehicle (Control) or IFN-γ (5,000 U/ml) in the presence or absence of the tested compound for 48 hours. Total RNA was isolated, and gene-specific mRNA concentrations were analyzed, using quantitative RT-PCR. Concentrations of EC-SOD and IRF-1 mRNA were normalized to the expression of GAPHD. The results shown represent the mean ± SD. *P < 0.001 and **P < 0.01, compared with cells treated with IFN-γ, and #P < 0.001, compared with cells treated with vehicle, according to ANOVA/Bonferroni post hoc test.