Figure 3.

N-methyl-4-isoleucine-cyclosporin (NIM811) blocks the CypA-induced migration of leukocytes in vitro. Purified populations of activated CD4⁺ T cells, neutrophils, and monocytes were set up in Boyden chemotaxis chambers in the presence of recombinant CypA ± 2 μM NIM811 (NIM) or diluent alone (DIL). Data show the mean chemotactic indices ± SEM for each group (n = 6 wells/group), as calculated by comparing the number of cells migrating in each test group relative to medium alone. A Student t test was used to establish statistically significant differences between NIM811-treated and DIL-treated groups. ***P < 0.0001.