Figure 5.

Inhibiting the function of cyclophilins during the chronic phase of asthma reduces tissue pathology after an acute reactivation of asthma. Lung pathology was analyzed in mice killed 24 hours after their second acute reactivation challenge, using the same timeline described in Figure 4. (A) Whole lungs from PBS-treated, diluent-treated, and NIM811-treated mice were fixed and embedded in paraffin, and 6-μm sections were cut and stained with periodic acid-Schiff (PAS) (left) or Masson's Trichrome (right). Black arrows denote areas of inflammatory foci. Images are shown at ×10 magnification. Scale bar = 10 μm. (B) Changes in the deposition of mucus and collagen were scored, using a semiquantitative scale ranging from 0 (normal) to 4 (pronounced deposition of mucus or collagen). A Student t test was used to identify significant differences between NIM-treated and DIL-treated groups (n = 6–8 mice per group). ***P < 0.0001.