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. 2011 Nov;45(5):923–930. doi: 10.1165/rcmb.2011-0032OC

Figure 3.

Figure 3.

Effects of overexpression of E2F1 on inhibition by NHE1 siRNA of human PASMC proliferation. (A) Expression of E2F1. Protein was isolated from human PASMCs under conditions of normoxia, hypoxia, and hypoxia, and treated with NHE1 siRNA for Western blot analysis. Left, representative imaging. Right, quantitative data. *P < 0.05, compared with other groups. #P > 0.05, compared with normoxia. Cell proliferation data were obtained through direct cell counts (B) and the BrdU incorporation assay (C). After transfection with NHE1 siRNA or E2F1 cDNA, PASMCs were cultured in a hypoxia chamber for 24 hours and harvested for cell counts and a BrdU assay. *P < 0.05, compared with the control group (n = 9 for each group). (D) Quantitative data on the protein expression of NHE1 and E2F1. Proteins isolated from PASMCs treated with NHE1 siRNA and E2F1 cDNA were subjected to Western blot analysis, setting the control value as 1. Data were normalized to Glyceraldehyde-3 phosphate dehydrogenase (GAPDH). *P < 0.05, compared with each control group (n = 3 for each group). NHE1 + E2F1, NHE1 siRNA + E2F1 cDNA.