Figure 6.

Effects of silencing NHE1 on proliferation of human (HPASMCs), human pulmonary artery endothelial cells (HPAECs), and human pulmonary artery fibroblasts (HPAFs) under condition of hypoxia. (A) Representative RT-PCR data show expression of NHE1 mRNA in three cell types. RNA was isolated from HPASMCs, HPAECs, and HPAFs. RT-PCR was performed to measure the expression of NHE1. GAPDH was used as loading control. (B) Cell proliferation data. After transfection with NHE1 siRNA, HPASMCs, HPAECs, and HPAFs were cultured in a 2% oxygen chamber for 24 hours, and harvested for gene expression and cell counts to assay cell proliferation. *P < 0.05, compared with control group (n = 9 for each group).