Table 1. RT-PCR primer sequences and amplification conditions.

Primer sequences (5′→3′)		Length	PCR setting
		(bp)	Denature	Annealing	Extension
β-catenin	5′-GCCAAGTGGGTGGTATAGAG-3′	330	95°C	53°C 40 s	72°C
	5′-GCTGGGTATCCTGATGTGC-3′		40 s	40 cycles	40 s
Kaiso	5′-TGCCTATTATAACAGAGTCTTT-3′	116	95°C	55°C 40 s	72°C
	5′-AGTAGGTGTGATATTTGTTAAAG-3′		40 s	40 cycles	40 s
cyclin D1	5′-CCCGATGCCAACCYCCTCAA-3′	500	95°C	58°C 40 s	72°C
	5′-TGGCACAGAGGGCAACGAAG-3′		40 s	40 cycles	40 s
cyclin E	5′-CTGGATGTTGACTGCCTTGA-3′	359	95°C	55°C 40 s	72°C
	5′-CCGCTGCTCTGCTTCTTAC-3′		40 s	40 cycles	40 s
cyclin D1	5′-CCCTCTCATGTAACCACGAA-3′	179	95°C	58°C 40 s	72°C
promoter	5′-TGGTTTTGTTGGGGGTGTAG-3′		40 s	40 cycles	40 s
β-actin	5′-AGAGCTACGAGCTGCCTGAC-3′	308	95°C	55°C 40 s	72°C
	5′-AGTACTTGCGCTCAGGAGGA-3′		40 s	40 cycles	40 s
GAPDH	5′-AAGGCTGGGGCTCATTTGCAG-3′	166	95°C	58°C 40 s	72°C
	5′-GGCCAGGGGTGCTAAGCAGTT-3′		40 s	40 cycles	40 s