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. 2012 Jan 20;7(1):e30403. doi: 10.1371/journal.pone.0030403

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Türck, Bierbaum.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Equal amounts of YycG-C-His₆ and YycG(Y306N)-C-His₆ ULVs as determined by the concentration of the inorganic phosphate (in triplicate) were incubated at different temperatures to observe changes in activity due to changes in microviscosity of the ULVs. All phosphorylation reactions were started by the addition of 4 µCi [γ-³³P]ATP and stopped after 60 min.