Table 1. Oligonucleotide primers used in this study.
| Primer | Sequence (5′→3′) | Annealing Temp. | Reference |
| a) Oligonucleotides used for cloning of the yyc genes into the expression plasmid pET22bΔpelB: | |||
| vicR-For | AAACCATGGGTATGCAAATGGCTAG (NcoI)* | 70.1°C | this work |
| vicR-Rev | CGACTCGAGCTCATGTTGTTGGAGG (XhoI) | 70.1°C | this work |
| vicK-For | GGTCCATGGGAATGAAGTGGCTAAA (NcoI) | 65.5°C | this work |
| vicK-Rev | TCCCTCGAGTTCATCCCAATCACCG (XhoI) | 65.5°C | this work |
| yycH-For | CACCCATGGGTGTGAAGTCATTGAA (NcoI) | 70.1°C | this work |
| yycH-Rev | GTTCTCGAGTTCAAGCCTCCCATCG (XhoI) | 70.1°C | this work |
| yycI-For | GGCCCATGGGAATGAACTGGAAACT (NcoI) | 53.0°C | this work |
| yycI-Rev | TTCCTCGAGATGATTAATAATTTTA (XhoI) | 53.0°C | this work |
| b) Oligonucleotides used for sequencing of pET22bΔpelB-derived plasmids: | |||
| T7 promoter | TAATACGACTCACTATAGG | ND | Novagen |
| T7 terminator | GCTAGTTATTGCTCAGCG | ND | Novagen |
| vicK innen | GCTTTAGCATTTAATAACTTGTCTA | ND | this work |
| c) Oligonucleotides used for cloning of groESL into pREP4groESL(MT): | |||
| groESL-For | CTTGCTAGCAGGAGAGTTATCAATGAATATTCG (NheI) | 57.3°C | this work |
| groESL-Rev | GCAGTCGACTTACATCATGCCGCCCATG (SalI) | 57.3°C | this work |
| d) Oligonucleotides used for sequencing of groESL harboured on pREP4groESL(MT): | |||
| pREP4_groESL-For | CACATATTCTGCTGACGCACCGGTG | ND | this work |
| pREP4_groESL-Rev | AATCCGTCGGCATCCAGGAAACCAG | ND | this work |
| groESL_innen | AACTGATCGCTGAAGCGATGGACAA | ND | this work |
*Restriction sites are indicated in brackets and underlined in the sequence. ND = Not determined.