Skip to main content
. 2012 Jan 20;7(1):e30764. doi: 10.1371/journal.pone.0030764

Figure 6. A putative HRE site is required for PDGF-BB activation of Mcl-1 promoter.

Figure 6

(A) The effects of PDGF-BB on the HIF-1 reporter activity in ARCaPM cells. The cells were transiently transfected with HIF-1 reporter or pGL3 for 24 h, serum-starved and incubated in the presence or absence of PDGF-BB (20 ng/ml) for 48 h. (B) Schematic diagram of human Mcl-1 promoter and its deletion mutation at the putative HRE site. (C) The effects of deleting the putative HRE site on PDGF regulation of Mcl-1 promoter activity in ARCaPM cells. IL-6 (200 ng/ml) was included as the positive control. (D) ChIP-Re-ChIP assay of the effects of PDGF-BB treatment (20 ng/ml) on the binding of β-catenin and HIF-1α to human Mcl-1 promoter region in ARCaPM cells.