Table 1. Primers used for single cell qPCR analysis.

Gene name and accession number	Primer name	Primer sequence (5′→3′)
Hprt	Hprt RT	CAAGGGCATATCCAACAACA
NM_013556	Hprt F	GGGGGCTATAAGTTCTTTGCT
	Hprt R	GGCCTGTATCCAACACTTCG
Cxcr4	CXCR4 RT	GACAAAGAGGAGGTCAGCCA
NM_009911	CXCR4 F	GTGCAGCAGGTAGCAGTGAA
	CXCR4 R	GGGTTCCTTGTTGGAGTCATAG
Vecad	VECad RT	CGGAGGGTTGTCATTCTCAT
NM_009868	VECad F	TGGTCACCATCAACGTCCTA
	VECad R	GCACAATGGACTCTTTCCCTAC
Ncad	NCad RT	AGGGTCTCCACCACTGATTCT
NM_007664	NCad F	ATGATCCAAATGCCCTGAAT
	NCad R	TTTGTCCGTGACAGTTAGGTTG
Casr	CASR RT	ATCCTGCCTGTGATGTTACG
NM_013803	CASR F	TATCCCCCAGGTGAGCTACG
	CASR R	GATCACTTCCACCACCTGCT
α4 integrin	VLA4 RT	CTTGAGAGGCGATCCACAT
NM_010576	VLA4 F	CACTCCAGCCGATCCTTCA
	VLA4 R	TGCAGGCAAGCTTCACTATG
α5 integrin	VLA5 RT	ACCTCCTGAGGTCTCCCATC
NM_010577	VLA5 F	ATCCTGTCCGCCACTCAA
	VLA5 R	GGTCATCTAGCCCATCTCCA
Lfa1	LFA1 RT	CCAGCGTCATTCCCAAGTA
NM_008400	LFA1 F	AGAAGCCACCATTTCCCTCT
	LFA1 R	TGCTTGTTCGGCAGTGATAG
Psgl1	PSGL1 RT	GTAGGGTCAGTGGTGGCAAT
NM_009151	PSGL1 F	CTGTCACTGAGGCAGAGTCGTT
	PSGL1 R	GTTCCCGGAGATGCACAG

Note: RT primers were used for retrotranscription reaction. Forward (F) and Reverse (R) primers were used for pre-amplification and qPCR reactions. Gene accession number for each gene obtained from NCBI database (http://www.ncbi.nlm.nih.gov/nuccore) [53].