Table 1.

Thermodynamic parameters describing allostery in AAC(6′)-Ii

K′A1 (×104 M−1)a	2.1	±0.1
K′A2 (×104 M−1)b	2.8	±0.1
K′U0c	0.20	±0.06
K′U1d	0.38	±0.07
ΔG′A1 (kcal mol−1)e	−6.13	±0.03
ΔG′A2 (kcal mol−1)f	−6.32	±0.02
ΔG′U0 (kcal mol−1)g	1.0	±0.2
ΔG′U1 (kcal mol−1)h	0.6	±0.1
ΔH′A1 (kcal mol−1)i	−22.9	±0.8
ΔH′A2 (kcal mol−1)j	−28.1	±0.8
ΔH′U0 (kcal mol−1)k	−71	±7
ΔH′U1 (kcal mol−1)l	−93	±6
ΔCp,A1 (cal mol−1 K−1)m	−790	±40
ΔCp,A2 (cal mol−1 K−1)n	−820	±30
ΔCp,U0 (cal mol−1 K−1)o	2100	NA
ΔCp,U1 (cal mol−1 K−1)o	2100	NA
[⊝] ′F (deg cm2 dmol−1)p	−7600	±200
[⊝] ′U (deg cm2 dmol−1)q	−4040	±20
mF (deg cm2 dmol−1 K−1)r	54	±3
mU (deg cm2 dmol−1 K−1)s	8	±2

T′=37°C

^aEquilibrium association constant for the native (non-melted) enzyme and the first AcCoA molecule at T′

^bEquilibrium association constant for the native (non-melted) enzyme and the second AcCoA molecule at T′

^cEquilibrium constant for subunit melting in the 0-bound enzyme at T′

^dEquilibrium constant for melting of the unbound subunit in the 1-bound enzyme at T′

^eFree energy change upon binding of one molecule of AcCoA to the native (non-melted) 0-bound enzyme at T′

^fFree energy change upon binding of one molecule of AcCoA to the native (non-melted) 1-bound enzyme at T′

^gFree energy change upon melting of a single subunit in the 0-bound enzyme at T′

^hFree energy change upon melting of the unbound subunit in the 1-bound enzyme at T′

ⁱEnthalpy change upon binding of one molecule of AcCoA to the native (non-melted) 0-bound enzyme at T′

^jEnthalpy change upon binding of one molecule of AcCoA to the native (non-melted) 1-bound enzyme at T′

^kEnthalpy change upon melting of a single subunit in the 0-bound enzyme at T′

^lEnthalpy change upon melting of the unbound subunit in the 1-bound enzyme at T′

^mHeat capacity change upon binding of one molecule of AcCoA to the native (non-melted) 0-bound enzyme

ⁿHeat capacity change upon binding of one molecule of AcCoA to the native (non-melted) 1-bound enzyme

^oHeat capacity change upon subunit melting. These values were fixed at the value obtained for a monomeric mutant of AAC(6′)-Ii

^pCD molar ellipticity of a native (non-melted) subunit at T′

^qCD molar ellipticity of a melted subunit at T′

^rTemperature dependence of the molar ellipticity of a native subunit

^sTemperature dependence of the molar ellipticity of a melted subunit