TABLE 1.

CYP-Mediated Formation of AAIa Form AAI in Human Hepatic Microsomes

CYP-dependent catalytic activitiesa
No.	pmol CYP per mg protein	Sudan I oxidation in presence of ketoconazole (CYP1A1)b	Phenacetin O-deethy-lation (CYP1A2)	Coumarin 7-hydroxylation (CYP2A6)	(S)-Mephenytoin N-demthy-lation (CYP2B6)	Paclitaxel 6α-hydroxy-lation (CYP2C8)	Diclofenac 4′-hydroxy-lation (CYP2C9)	(S)-Mephenytoin 4′-hydroxy-lation (CYP2C19)	Burfuralol 1′-hydroxy-lation (CYP2D6)	Chloroxazone 6-hydroxy-lation (CYP2E1)	Testosterone 6β-hydroxy-lation (CYP3A4)	Lauric acid 12-hydroxy-lation (CYP4A)	AAIs Peak area
HG93	290	1.1	750	340	25	180	2100	41	56	1800	2400	1600	6.0
HG03	290	0.9	170	2000	51	200	1700	44	110	1800	6100	1600	2.0
HG74	220	1.2	520	360	13	130	2100	55	120	1400	2700	1300	7.8
HG06	300	0.9	770	580	3.0	130	2650	36	ND	1200	2990	740	5.8
HK27	300	1.6	1320	1320	31	180	480	460	130	3000	4910	1110	10
HG42	670	0.9	700	2200	150	480	1600	7.0	95	1600	15,000	1400	4.0
HG112	560	0.5	320	490	150	180	4000	380	32	2400	25,000	1800	2.7
HG56	550	3.0	2400	1500	48	180	3100	480	120	1900	5300	3000	18
HG43	270	1.2	630	850	34	60	1400	700	20	1100	5600	2100	3.8
HG89	400	1.6	1500	650	60	160	2100	190	13	1700	12,000	2200	16
HG32	170	1.3	730	520	1.0	20	450	5.0	46	1200	2000	680	4.8
HK31	580	2.5	1220	2160	8.0	130	1690	170	3.0	1660	8210	2010	17.5
HK23	380	1.6	960	1100	24	160	2100	110	140	2100	6800	780	7.2
HK34	500	1.6	1000	1500	39	220	1900	45	100	6000	5200	1100	6.15
Meansc	390 (150)	1.4 (0.6)	930 (540)	1110 (650)	46 (46)	170 (100)	1960 (890)	200 (210)	76 (47)	2060 (1200)	7440 (6010)	1530 (630)	8.0 (5.2)

^aCYP activities in picomoles per minute per milligram protein. CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4, and CYP4A determined by Gentest.

^bPicomole total C-hydroxylated metabolites per minute per milligram protein.

^cArithmetic means for hepatic microsomal samples of 14 human donors and (SD), representing interindividual variability.