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L. major infection induces increased CCR7 expression on bystander DCs in a TLR9-dependent manner. Bone marrow dendritic cell cultures from C57BL/6 (A, B) or TLR9−/− (C, D) mice were uninfected (A, C) or infected (B, D) at a 10:1 ratio with L. major for 2 hours and then incubated overnight. Cells were stained with fluorescent antibodies and results were assessed by flow cytometry. These results are representative of 3 experiments.
