Figure 7.
TLR9 expression in DCs is required and sufficient to induce LN hypertrophy. TLR9−/− DCs activated with L. major fail to induce LN hypertrophy. L. major infected DCs (5 × 105) from C57BL/6 (B6) or TLR9−/− mice, or uninfected DCs, were injected into the footpad of C57BL/6 mice, and the cellularity of the dLN assessed on day 3 (A). L. major infected DCs (1 × 106) from C57BL/6 (B6) or TLR9−/− mice, or uninfected DCs, were injected into the footpad of CD45.1 C57BL/6 mice, and the presence of the donor cells in the dLN assessed on day 3 (B). L. major infected DCs (1 × 106) from C57BL/6 (B6), or uninfected DCs, were injected into the footpad of C57BL/6 mice or TLR9−/− mice, and the cellularity of the dLN assessed on day 3 (C). The results expressed are the mean (+/− SE) number of cells in the dLN of 3 mice per group. The results are representative of 2 experiments.