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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Immunol. 2011 Dec 19;188(3):1206–1212. doi: 10.4049/jimmunol.1102773

Figure 5. SAP knockdown abrogates IL-17 production.

Figure 5

Naïve CD4+ T lymphocytes were transfected with SAP siRNA or control siRNA as indicated. Efficacy of siRNA transfection was determined by SAP protein levels using western blot analysis (A). Three days after transfection cells were co-stimulated with anti-SLAMF3 (B) or anti-SLAMF6 antibodies (C) in addition to anti-CD3 under Th17 differentiation conditions for another 4 days. Levels of secreted IL-17 were measured by ELISA.