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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Nat Immunol. 2012 Jan 8;13(2):136–143. doi: 10.1038/ni.2205

Figure 3. Foam cell secreted netrin-1 blocks macrophage migration.

Figure 3

(a-c) Migration of pMø to CCL19 (500 ng/ml) with/without netrin-1 (250 ng/ml) in the presence of (a) recombinant UNC5b-Fc or IgG control, (b) anti-UNC5b or isotype-matched control antibody, or (c) an inhibitor of the A2B adenosine receptor (10 uM 8-PT). (d-e) Migration of pMø to CCL19 (500 ng/ml) in (d) the presence or absence of conditioned medium (CM) from macrophages treated with LDL or oxLDL (50 μg/ml; 48 h) and (e) with/without recombinant UNC5b-Fc or IgG control. (f) Migration of pMø to CCL19 (500 ng/ml) as in (e) except using conditioned medium (CM) from WT or Ntn1−/− macrophages treated with oxLDL. Inset: immunoblot of netrin-1 in lysates of WT and Ntn1−/− pMø. (a-f) Data are the mean ± s.d. of triplicate samples in a single experiment and are representative of 3 independent experiments. *P < 0.01. (g) Effect of netrin-1 (500ng) or PBS pretreatment on LPS-induced leukocyte emigration from the peritoneum of mice with established thioglycollate-induced peritonitis. Data are the mean number (± s.e.m) of leukocytes in the peritoneum of mice (n = 6/group) before and 4h after injection of 400ng LPS ip., and are representative of 2 independent experiments. *P < 0.05.