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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Nat Immunol. 2011 Dec 25;13(2):129–135. doi: 10.1038/ni.2203

Fig. 1. β1i and β5i double KO mice.

Fig. 1

(a) Exons targeted for the sequential KO of both Psmb9 and Psmb8 to generate double deficient mice. β1i and β5i proteins are not detected in spleen cell lysates (b) or proteasomes purified from splenocytes (c) (equivalent protein loaded in each lane). (d) The amount of proteasomes in WT and TKO spleens is similar, as measured by alpha subunits in serial dilutions of proteasome pellets from equal numbers of spleens. (e) Normalized TAP1 mRNA expression in WT and β5iβ1i double KO splenocytes, determined by RT-qPCR. Bar indicates mean +/− SD of 5 animals for each strain. Asterisk indicates P < 0.05 (Two-tailed, unpaired t-test).(f) Immunoblotting of splenocyte whole cell lysates from the indicated strains, using anti-TAP1 antibody (equivalent protein loaded in each lane).