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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Immunol. 2011 Dec 16;188(3):1381–1393. doi: 10.4049/jimmunol.1102359

Figure 2. IL-10 expression in vivo is found in both CD4+ T cells and macrophages.

Figure 2

The IL-10 GFP reporter mouse strain designated ‘tiger’ was used to identify cellular sources of IL-10 in the joint in vivo. Flow cytometry data in A–E utilized primarily lymphoid markers to designate populations, while date from F–G represent a separate myeloid analysis. Gate boundaries for GFP and lineage markers were established by isotype control staining in cells derived from non-tiger mice. Data from A-iii, vi, and B–G were acquired from a single infected tiger mouse at 2 weeks post infection. Analysis of 3 infected tiger mice yielded similar results. IL-10 (GFP) expression in CD45+ cells from infected tiger mice, in both the lymph nodes (A-iii) and joint tissue (A-vi). IL-10 (GFP) expression in the tissues of non-tiger or uninfected tiger mice (Ai, ii, iv, v). Analysis of lineage-specific surface markers that were present on IL-10 (GFP)+ cells in infected lymph nodes (B). Frequency of CD4+, TCR-β+ T cells in the total IL-10 (GFP)+ cells in lymph node tissue (C). Analysis of lymphocyte lineage-specific surface markers that were present on IL-10 (GFP)+ cells in infected joint tissue (D). Frequency of CD4+, TCR-β+ T cells in the total IL-10 (GFP)+ cells in infected joint tissue (E). Analysis of myeloid lineage-specific surface markers that were present on IL-10 (GFP)+ cells in infected joint tissue (F). Frequency of CD11b+, F4/80+ macrophages in the total IL-10 (GFP)+ cells in infected joint tissue (G).