Induction of Th1 and Th17 cytokine responses after DSS treatment. Time course of cytokine protein levels from the colons of WT (dark rectangles) and PSGL-1−/− (open rectangles) mice after DSS treatment. Colons were removed at baseline, day 7, and day 14, opened, washed, and the entire colon was cultured in 6 wells of a 24-well plate overnight for direct cytokine analysis of supernatents by ELISA (n = 4 per group). *, p < 0.05; #, p < 0.01 vs. WT, same day. Data are mean ± SEM from duplicate samples.