Induction of Th1 cytokine and chemokine responses after DSS treatment. Time course of mRNA levels from the colons of WT (dark rectangles) and PSGL-1−/− (open rectangles) mice after DSS treatment. Colons were removed at baseline, day 7, and day 14, opened, washed, and 0.5 cm of distal colon was preserved in RNAllater for subsequent isolation of mRNA and real time PCR from duplicate samples (n = 4 per group). *, p < 0.05; #, p < 0.01 vs. WT, same day. Data are mean ± SEM.