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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Neuroendocrinol. 2012 Apr;24(4):528–538. doi: 10.1111/j.1365-2826.2011.02236.x

Figure 5.

Figure 5

Use of Adeno-Associated Virus (AAV) to study cell-type specific expression in (A) Avp and (B) Oxt MCNs in vivo. Stereotaxic injections of rAAV vectors containing construct with either Avp (in A) or Oxt (in B) promoters fused to EGFP reporters produced cell-specific expression of the EGFP in the injected SONs. A. Shows EGFP expression in the MCNs, which co-localize with Avp-immunoreactivity (Avp-ir), but not with Oxt-immunoreactivity (Oxt-ir). B. Shows EGFP expression in the MCNs, which co-localize with Oxt-ir, but not with Avp-ir. The IGR is the intergenic region sequence as defined in Fields et. al.(2003; ref 50). For the AVP construct the IGR was 181bp, for the OXT construct the IGR was 442bp. The ITRs (Inverted Terminal Repeat Sequences) are necessary for viral replication, rescue, packaging and integration (see Fig. 4 top).