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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Immunol. 2012 Jan 4;188(3):957–967. doi: 10.4049/jimmunol.1100461

Figure 1. SP-A can either enhance or suppresses exogenous stimulation induced T cell activation in the lung in vivo.

Figure 1

Mice were oropharyngeally instilled with low (10 ng/mouse) or high (1000 ng/mouse) LPS in conjunction with CFSE. Single cell suspensions from spleen and lung digests were prepared after 68-72 h, stained with T cell markers and analyzed for CFSE-int (divided cells) by flow cytometry (1A-B). Data is normalized to WT spleen, and represented as the fold difference in CFSE-int T cells from either 8 or 9 mice per group, with three independent experiments. Proliferating BrdU+ T cells in lungs or spleen were identified by intracellular flow cytometric staining in conjunction with surface markers. * p<0.05 compared to WT mice.