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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Immunol. 2011 Dec 28;188(3):1451–1459. doi: 10.4049/jimmunol.1102705

Figure 2. Evidence of cellular IFN-I activation, yet normal lymphocyte homeostasis in MDA5 Tgs.

Figure 2

(A) Ly6C and Ly6A/E surface expression was evaluated by flow cytometry on CD8+, B220+ CD4+ and CD11c+ cells from WT and MDA5 Tg spleens or bone marrow. (B) PDCA-1 surface expression levels were evaluated on B cells and cDCs in the spleen as well as cDCs and monocytes in the bone marrow of WT and MDA5 Tg mice. (C–E) Splenocytes from MDA5 Tg and WT littermate controls were gated by flow cytometry using the indicated markers. Each dot represents absolute numbers of cells from an individual mouse. Mice were between 2 and 6 months of age with little age-dependent differences observed. A statistical T-test was performed comparing WT to MDA5 Tg. **P<0.01. (F) Representative dot plots of Ly6C staining on monocytes with a gate showing the frequency of CD11b+Ly6CHi cells. In A, B and F, representative cytometric plots from at least three mice in each group are shown.