Skip to main content
. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Immunol. 2011 Dec 28;188(3):1451–1459. doi: 10.4049/jimmunol.1102705

Figure 5. The MDA5 Transgene enhances B cell responses in FcγR2B−/− mice.

Figure 5

(A) 3–6 month old WT, MDA5 Tg, FcγR2B−/− and MDA5 Tg FcγR2B−/− mice were analyzed for the presence of class-switched (B220+IgG2a+), GC (B220+FAS+GL7+) and plasma blast (B220intCD138+) B cells using flow cytometry. Additionally, data points for WT and MDA5 Tg animals from Figure 2 are plotted here for comparison. Absolute numbers for individual mice are displayed. (B) Mean fluorescence intensity of ICOS and numbers for CD4+CD44HiCXCR5+ T follicular helper cells were measured on splenocytes purified from mice of the indicated genotype. Each dot represents an individual mouse. (C) Total IgG, IgG1 and IgG2a was measured in the sera of 2–4 month old WT, MDA5 Tg, FcγR2B−/− and MDA5 Tg FcγR2B−/− mice by ELISA. Each dot represents a single mouse. *P<0.05, **P<0.01, ***P<0.001.