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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Immunol. 2011 Dec 28;188(3):1451–1459. doi: 10.4049/jimmunol.1102705

Figure 6. The MDA5 Transgene accelerates autoimmunity in FcγR2B−/− mice.

Figure 6

(A) Cumulative survival was monitored for FcγR2B+/−, MDA5 Tg FcγR2B+/−, FcγR2B−/− and MDA5 Tg FcγR2B−/− mice for 10 months (left panel). The level of proteinuria was measured in the urine using a dip stick (Right Panel). Data is represented as the percentage of mice that are proteinuria free throughout the 10-month period. (B) Spleen weights from 2–4 month FcγR2B−/− and MDA5 Tg FcγR2B−/− mice were quantified. Spleen weights for WT and MDA5 Tgs from Figure 1D are plotted for comparison. (C) Total IgG + IgM anti-nuclear antibody levels were quantified using an ANA ELISA. (D) Kidneys from 2.5–4 month old mice of the indicated genotype were stained with H&E and kidney sections were analyzed for glomerulonephritis. Glomeruli score was determined by measuring individual glomeruli from multiple fields of view. WT n=3 mice, FcγR2B−/− n=6 mice and MDA5 Tg FcγR2B−/− n=8 mice. For A–D each dot represents a single mouse. Data are pooled from multiple experiments. For A, Log-rank (Mantel-Cox) and Gehan-Breslow-Wilcoxon tests were performed with similar P values. *P<0.05, **P<0.01, ***P<0.001.