Table 1.
Bioreactor Operating Parameters
| 800 mL bioreactor | 8 mL bioreactor | 2 mL bioreactor | |
|---|---|---|---|
| Cell number inoculated CD34+ HSCs | 8×107 (theoretical) | 8.0×105 1.8×106 (actual) | 8×105 (actual) |
| Circulating volume (mL) | 1200 | 50 | 25 |
| Medium recirculation rate (mL/min) | 250 | 20 | 15 |
| Medium feed rate (mL/h) | 150 | 1–4 | 1 |
| Air flow rate (mL/min) | 550–600 | 30–50 | 30–50 |
| CO2 flow rate (mL/min) | 10–12 | 3–5 | 1–2 |
| Average pressure in the cell compartment (mmHg) | 40 | 10 | 5 |
| Medium pH value | 7.35–7.45 | 7.35–7.45 | 7.35–7.45 |
| Temperature (°C) | 38 | 38 | 38 |
Bioreactor perfusion conditions in 800, 8 or 2 mL hollow-fiber scale bioreactors using HSCs.
HSCs, hematopoietic stem cells.