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. 2012 Jan 4;32(1):124–132. doi: 10.1523/JNEUROSCI.3442-11.2012

Figure 2.

Figure 2.

SIRT1 decreases α-synuclein aggregates in A53T mouse brain. A, Immunostaining of α-synuclein aggregates in cortical sections of 3.5-month-old A53T and A53T-Tg mice (top panel) and A53T-F/F and A53T-BSKO mice (middle panel). Quantification is shown on the right. n = 6 for each genotype. There were no α-synuclein aggregates observed in wt mice or proteinase K-treated cortical sections of A53T mice (bottom panel, left and middle, respectively). Higher magnification picture of α-synuclein aggregates is also demonstrated (bottom panel, right). The statistical analysis was performed using Student's t test, and significant differences are demonstrated by a single asterisk (*) indicating p < 0.01. Error bars in figures represent SEM. B, Immunostaining of gliosis in cortical sections of A53T, A53T-Tg, and wt mice (top and middle panels) and A53T-F/F and A53T-BSKO mice (bottom panel) is performed by using GFAP antibody. Quantification is shown on the right. n = 6 for each genotype. The statistical analysis was performed by two-way ANOVA in the top panel, and significant differences are demonstrated by single asterisk (*) or pound sign (#) indicating p < 0.01. In the bottom panel, Student's t test was performed for statistical analysis; significant differences are demonstrated by single asterisk (*) indicating p < 0.01. Error bars in figures represent SEM.