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. 2012 Jan 4;32(1):124–132. doi: 10.1523/JNEUROSCI.3442-11.2012

Figure 6.

Figure 6.

SIRT1 deacetylates HSF1 and activates Hsp70 in A53T α-synuclein-expressing H4 cells. Left, Lysates from A53T-overexpressing H4 cells transfected by SIRT1 (mSIRT1), SIRT1-shRNA, or vector immunoprecipitated with anti-HSF1 antibody or NRS and blotted with anti-HSF1 or anti-pan-acetylated lysine (Ac-K) antibodies. Right, Hsp70 RNA levels quantified from cell lysates of A53T-overexpressing H4 cells transfected by SIRT1 (mSIRT1), SIRT1-shRNA, or vector quantified by qPCR. The analysis was performed using two-way ANOVA, and significant differences are demonstrated by single asterisk (*) or pound sign (#) indicating p < 0.01. Error bars in figures represent SEM.