IL-4/eGFP reporter mice were treated with PBS or rTSLP and splenic basophils were (a) identified and (b) quantified. WT mice were treated with control-cDNA or TSLP-encoding (TSLP-cDNA) plasmid and splenic basophils were (c) identified and (d) quantified. Serum (e) IL-4, (f) IL-5, (g) IL-13 and (h) total IgE were quantified. (i), Histologic analysis of small intestine. Scale bars, 100 μm. (j), Splenic basophils from control mice (WT), or (SPC-TSLP) mice were identified (j) and (k) quantified. Results are representative of at least three (a-d and j-k) or two (e-i) separate experiments containing 3-5 (a-d), 5 (e-i) or 6-12 (j-k) mice per group. Statistical analyses performed using a two-tailed students t test (*, p<0.05), (**, p<0.01).