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. Author manuscript; available in PMC: 2012 Mar 8.
Published in final edited form as: Nature. 2011 Aug 14;477(7363):229–233. doi: 10.1038/nature10329

Figure 2. TSLP preferentially expands basophil populations from bone marrow- resident cells.

Figure 2

Bone marrow-resident cells were taken from IL-4/eGFP reporter mice and cultured in the presence of (a) media, (c) IL-3 or (f) TSLP, and the frequency of IL- 4/eGFP+ NBNT cells was determined. (b, d, g), The frequencies of IL-4/eGFP+, NBNT, Siglec-F- basophils and mast cells were determined. Cytology of IL-4/eGFP+, CD49b+, FcεRI+, c-Kit- (e) IL-3-elicited or (h) TSLP-elicited basophils. Results are representative of at least three separate experiments. Italicized numbers in basophil gates represent the MFI levels of CD49b staining. Scale bar, 10 μm.