Bone marrow-resident cells were taken from IL-4/eGFP reporter mice and cultured in the presence of (a) media, (c) IL-3 or (f) TSLP, and the frequency of IL- 4/eGFP+ NBNT cells was determined. (b, d, g), The frequencies of IL-4/eGFP+, NBNT, Siglec-F- basophils and mast cells were determined. Cytology of IL-4/eGFP+, CD49b+, FcεRI+, c-Kit- (e) IL-3-elicited or (h) TSLP-elicited basophils. Results are representative of at least three separate experiments. Italicized numbers in basophil gates represent the MFI levels of CD49b staining. Scale bar, 10 μm.