IL-3- elicited or TSLP-elicited murine basophils were stained (shaded) for (a), CD200R, CD69, CD11b, CD62L, (b) CD123, T1/ST2 and IL-18Rα expression and compared to fluorescence minus one (FMO) controls. Numbers represent MFI levels. Results are representative of at least 3 separate experiments. (c), GSEA of microarray data comparing IL-3-elicited and TSLP-elicited basophils. (d), Activated human basophils stained for TSLPR. (e), Representative histograms demonstrating ST2 expression (shaded) compared to isotype controls. (f), Percentage of basophils from control or EoE patients expressing ST2 compared to isotype controls. Statistical analysis was performed using a two-tailed students t test.