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. Author manuscript; available in PMC: 2013 Jan 5.
Published in final edited form as: Toxicol Lett. 2011 Oct 6;208(1):51–61. doi: 10.1016/j.toxlet.2011.09.030

Figure 3. Effect of harmine and harmol on CYP1A1 mRNA, protein, and catalytic activity in HepG2 cells.

Figure 3

Cells were incubated with increasing concentrations of harmine or harmol (0.5-12.5 μM) 30 min before the addition of TCDD (1nM) for an additional 6 h for mRNA or 24 h for protein and catalytic activity. A, The amount of CYP1A1 mRNA was quantified using real-time PCR and normalized to β-actin housekeeping gene. Values represent the mean of fold change ± S.E.M. (n=4). B, Protein was separated on a 10% SDS-PAGE and CYP1A1 protein was determined using the enhanced chemiluminescence method. The intensity of bands was normalized to GAPDH signals, which was used as loading control. One of three representative experiments is shown. C, CYP1A1 activity was determined using CYP1A1-dependent EROD assay. Values represent mean activity ± S.E.M. (n = 8). (+) P < 0.05 compared with Control (C), (*) P < 0.05 compared with TCDD (T), (L); ligand.