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. 1983 Sep 10;11(17):6079–6087. doi: 10.1093/nar/11.17.6079

The structure of cloned 3'-terminal RNA region of bovine leukemia virus (BLV).

A Tsimanis, V Bichko, D Dreilina, J Meldrais, V Lozha, R Kukaine, E Gren
PMCID: PMC326336  PMID: 6310526

Abstract

cDNA synthesized on the bovine leukemia virus RNA template has been cloned in the pBR322 Pst I site. Colony hybridization with BLV RNA fragments and oligo (dT) has revealed a clone with cDNA insert containing 660 3'-terminal nucleotides of the BLV genome. The nucleotide sequence of the insert corresponding to U3 and R regions of the long terminal repeats (LTR) of viral genome has been determined. BLV U3, like U3 of other retroviruses, presumably contains promoter. The unusually long R region (about 230 bp), a certain homology with ATLV U3-R and some other structural features allow to group BLV LTR together with ATLV LTR in a separate class of retroviral LTR.

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Selected References

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