FIGURE 4.

Real-time PCR of B cell subsets. A, Ratio of VκRF to glD42H in sorted subset pellets. Negative selection of VκRF occurs at the immature to T1 stage, and positive selection occurs at the GC stage (^†p < 0.01 compared with previous B cell developmental stage). B, RAG-1 was detected in bone marrow B cells, but not in FO or GC B cells (similar results were obtained for RAG-2). Pellets from three individual mice were used per group, and experiments were repeated once.