Fig. 4.

Cell microarray incubated with HeLa cells demonstrates both lentiviral gene knockdown and gene expression on printed spots. Each spot is approximately 250 μm in diameter and contains ~150 cells. A. Cells in contact with a GFP lentivirus spot show effective GFP expression (circle at top right); cells in contact with shRNA against lamin A/C show loss of Cy3 lamin A/C signal (lower right). The circle at center shows HeLa cells attached to the slide but not touching a lentivirus spot. B. Quantitation of lamin A/C knockdown and GFP expression from cell microarrays. Scale bar, 1 mm (left panel) and 200 μm (right). Adapted from⁹⁶.