Fig. 5.
Miniaturized quantitative RT-PCR analysis enabled by microfluidic integrated fluidic circuits. A. Image of a 48 × 48 microfluidic chip. The integrated fluidic circuit (at center) contains 2304 reaction chambers (RC, outlined by a red box in inset), bounded by control valves (CV) and interface valves (IV). B. Heatmap of RT-PCR Ct values from individual reaction chambers on a single chip. C. Correlation between expression levels obtained by microfluidic (nanoliter scale) and conventional (microliter scale) quantitative RT-PCR. r = 0.986. Adapted from115.