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. 2012 Jan 1;2(1):76–85. doi: 10.7150/thno.3462

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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In vitro evaluation of cytokines release and nitric oxide production in macrophage. The levels of (A) IL-6 and (B) TNF-α released from mouse macrophage cells (RAW264.7). RAW264.7 cells were treated with TCL-SPION or MION-47 at concentrations of 25 µg Fe/mL and 50 µg Fe/mL for 24 hours. The IL-6 and TNF-α releases in the RAW264.7 cells were dose-dependently and significantly increased by adding TCL-SPION and MION-47 (*, P < 0.001, vs. control). MION-47 showed a greater stimulatory effect on IL-6 release than did TCL-SPION, while the TNF-α stimulatory effect was similar. (C) Nitric oxide production in macrophages. Macrophages were treated TCL-SPION or MION-47 at concentrations of 25 µg Fe/mL, 50 µg Fe/mL and 100 µg Fe/mL for 24 hours. The nitrite levels were assayed by the Griess reaction. MION-47 induced a dose-dependent and significant increase in nitric oxide production (*, P < 0.001, vs. control), but TCL-SPION did not have any stimulatory effect on nitric oxide production. All data are presented as the means ± standard errors from at least three independent experiments.