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. Author manuscript; available in PMC: 2013 Jan 2.
Published in final edited form as: FEBS Lett. 2011 Dec 9;586(1):102–106. doi: 10.1016/j.febslet.2011.12.005

Fig. 3. Transactivation of L1 promoter activity by Rb and HDAC2 is ablated by HPV E7 viral oncoprotein.

Fig. 3

Transient transcription assays in HeLa cells with the pBASIC-luciferase or the pL1Md-A5-luciferase reporter vectors and the forced overexpression of HPV E7, pRb, or HDAC2 proteins alone or in combination are shown. As an internal control for normalization, 10 ng of pRL were cotransfected within each experiment. (A) pBASIC-luciferase reporter activity corrected for pRL after cotransfection with 100 ng of empty vector control, or expression vectors indicated in the figure label. (B) pL1Md-A5-luciferase reporter activity corrected for pRL after cotransfection with 100 ng of empty vector control, or expression vectors indicated in the figure label. Data were normalized as indicated in the Y axis. Cells were transfected for 6 hours, allowed to recover for 30 hours prior to luciferase activity measurements. Results are expressed as arbitrary luciferase units (ALU) and represent the mean ± S.D. of triplicate experiments after normalization to pRL. Statistic analyses were done using ANOVA (*, **, and *** indicate statistically significant differences, p<0.05, p<0.005 and p<0.0005 respectively). Each experiment was repeated at least 2 times.