Figure 4.

Expression of p14^ARF, p16^INK4A, MGMT, and RASSF1A in astrocytoma cell lines determined by RT-PCR. PCR products were visualized in a 2% agarose gel stained with 0.5 μg/mL ethidium bromide. m: molecular weight marker: 1 Kb Plus DNA ladder; 1: U87MG; 2: A172; 3: H4; 4: GOS3; 5: SW1783; 6: SW1088; 7: CCF-STTG1; 8: MOG-G-CCM; 9: T98G; 10: LN 405; 11: SK-N-Be(2) (neuroblastoma); N: peripheral blood lymphocytes; 12: genomic DNA; 13: water. Cell lines A172 and H4 were not subjected to the methylation study. A fragment of the transferrin receptor gene (TFR) was reverse-transcribed as an internal control.