Table 2.
Lipophilic compounds found in femoral gland secretions of male lizards, P. hispanica, from five distinct populations of the Madrid region (Aranjuez, Belmonte, Golondrina, Fuenfría, and Pedrezuela). The relative amount of each component was determined as the percent of the total ion current (TIC) and reported as the average (±SE). Characteristics (m/z) are reported for some unidentified (Un.) compounds. (RT: retention time).
| Compounds | RT (min) | Fuenfría | Pedrezuela | Golondrina | Belmonte | Aranjuez |
|---|---|---|---|---|---|---|
| Steroids | ||||||
| Un. steroid (145,213,248,353,368,387) |
29.92 | 0.01 ± 0.01 | — | 0.17 ± 0.05 | 1.49 ± 0.56 | — |
| Cholesta-2-4-diene | 30.58 | 0.68 ± 0.11 | 2.66 ± 0.44 | 0.44 ± 0.08 | 2.59 ± 0.46 | 0.96 ± 0.35 |
| Cholesta-3.5-diene | 30.81 | 0.42 ± 0.10 | 0.23 ± 0.04 | 0.30 ± 0.07 | 0.13 ± 0.03 | 0.25 ± 0.07 |
| Un. steroid (155,197,251,350,365) | 30.96 | 1.32 ± 0.16 | 1.00 ± 0.14 | 0.55 ± 0 | 0.45 ± 0.06 | 0.45 ± 0.17 |
| Cholesta-5,7,9(11)-trien-3-ol | 31.06 | 1.62 ± 0.18 | 1.07 ± 0.24 | 0.94 ± 0.11 | 0.65 ± 0.11 | 0.29 ± 0.07 |
| Un. steroid (207,251,350,365) | 31.13 | 0.40 ± 0.08 | 0.16 ± 0.02 | 0.18 ± 0.02 | 0.18 ± 0.07 | 0.08 ± 0.04 |
| Un. steroid (143,195,207,351,366) | 31.20 | 0.19 ± 0.02 | 0.08 ± 0.01 | 0.15 ± 0.04 | 0.18 ± 0.05 | 0.22 ± 0.06 |
| Un. steroid (141,156,209,350,365) | 31.37 | 0.37 ± 0.05 | 0.03 ± 0.01 | 0.30 ± 0.06 | 2.47 ± 0.42 | — |
| Un. steroid (155,197,251,365,379) | 31.64 | 0.06 ± 0.01 | 0.21 ± 0.02 | 0.08 ± 0.02 | 0.43 ± 0.07 | 0.45 ± 0.18 |
| Un. steroid (195,209,251,365,379) | 31.84 | — | 0.07 ± 0.01 | 0.27 ± 0.07 | 0.51 ± 0.08 | 0.32 ± 0.12 |
| Cholesterol | 32.43 | 59.74 ± 2.79 | 62.33 ± 1.68 | 66.61 ± 2.00 | 53.03 ± 2.51 | 74.51 ± 2.04 |
| Cholestanol | 32.47 | 1.40 ± 0.14 | 0.53 ± 0.08 | 0.90 ± 0.11 | 0.60 ± 0.06 | 0.55 ± 0.12 |
| Cholesta-5.7-dien-3-ol. | 32.65 | 13.41 ± 1.85 | 2.68 ± 0.54 | 8.02 ± 1.33 | 1.16 ± 0.19 | 0.54 ± 0.17 |
| Un.steroid (105,213,255,353,368,386,415) | 32.75 | 0.02 ± 0.01 | 0.03 ± 0.02 | 0.35 ± 0.11 | 0.09 ± 0.03 | 0.39 ± 0.16 |
| Ergosterol | 33.00 | — | 0.05 ± 0.02 | — | 0.17 ± 0.11 | — |
| Campesterol | 33.17 | 1.61 ± 0.22 | 3.76 ± 0.28 | 3.27 ± 0.36 | 5.46 ± 0.28 | 4.22 ± 0.57 |
| Cholest-4-en-3-one | 33.41 | 0.17 ± 0.03 | 0.53 ± 0.17 | 0.19 ± 0.05 | 0.20 ± 0.02 | 0.92 ± 0.38 |
| Ergosta-5,8-dien-3-ol | 33.50 | 2.43 ± 0.30 | 1.58 ± 0.22 | 2.38 ± 0.37 | 1.31 ± 0.24 | 0.56 ± 0.14 |
| Cholesta-4,6-dien-3-one | 33.69 | 0.24 ± 0.06 | 0.53 ± 0.08 | 0.29 ± 0.06 | 0.40 ± 0.06 | — |
| Sitosterol | 33.92 | 0.65 ± 0.10 | 0.74 ± 0.16 | 0.94 ± 0.15 | 1.18 ± 0.11 | 1.13 ± 0.23 |
| Ergostanol | 34.02 | 0.07 ± 0.01 | 0.08 ± 0.03 | 0.10 ± 0.02 | 0.11 ± 0.02 | 0.33 ± 0.11 |
| Stigmasterol | 34.13 | 0.31 ± 0.06 | 0.27 ± 0.13 | 0.28 ± 0.04 | 1.22 ± 0.22 | 0.44 ± 0.26 |
| Un.steroid (221,253,281,355,380,430) | 34.30 | 2.23 ± 0.32 | 0.70 ± 0.18 | 1.01 ± 0.16 | — | — |
| Cholest-5-en-3-one | 34.38 | — | — | — | 1.33 ± 0.24 | 0.91 ± 0.28 |
| Ergosta-5.22-dien-3-ol | 34.47 | — | 0.13 ± 0.07 | 0.12 ± 0.03 | 0.15 ± 0.04 | — |
| Un.steroid (214,267,395) | 35.30 | 0.12 ± 0.04 | 0.21 ± 0.11 | — | 0.56 ± 0.44 | 0.22 ± 0.09 |
|
| ||||||
| Carboxylic acids and their esters | ||||||
| Tetradecanoic acid | 20.64 | 0.16 ± 0.04 | 0.38 ± 0.13 | 0.22 ± 0.06 | 0.24 ± 0.05 | 0.85 ± 0.55 |
| Pentadecanoic acid | 21.68 | 0.13 ± 0.02 | 0.15 ± 0.12 | 0.10 ± 0.03 | 0.18 ± 0.05 | 0.41 ± 0.19 |
| Hexadecanoic acid. methyl ester | 22.33 | — | 0.05 ± 0.02 | — | 0.09 ± 0.02 | 0.25 ± 0.08 |
| Hexadecenoic acid | 22.54 | 0.16 ± 0.02 | 0.40 ± 0.20 | 0.25 ± 0.07 | 0.57 ± 0.33 | 0.28 ± 0.09 |
| Hexadecanoic acid | 22.76 | 3.68 ± 0.32 | 4.36 ± 0.65 | 3.11 ± 0.35 | 5.98 ± 0.51 | 1.54 ± 0.23 |
| Hexadecanoic acid, ethyl ester | 22.98 | — | 0.37 ± 0.11 | — | 0.19 ± 0.06 | 0.40 ± 0.17 |
| 9,12-octadecadienoic acid | 24.35 | 0.10 ± 0.01 | 0.11 ± 0.02 | 0.12 ± 0.02 | 0.27 ± 0.08 | 0.06 ± 0.02 |
| Octadecenoic acid | 24.43 | 1.99 ± 0.18 | 1.76 ± 0.20 | 2.76 ± 0.57 | 4.82 ± 1.41 | 1.01 ± 0.21 |
| Octadecanoic acid | 24.60 | 1.39 ± 0.12 | 2.52 ± 0.34 | 1.41 ± 0.13 | 2.55 ± 0.23 | 0.99 ± 0.18 |
| Octadecanoic acid, ethyl ester | 24.82 | — | 0.51 ± 0.23 | — | 0.14 ± 0.04 | 0.55 ± 0.23 |
| Eicosanoic Acid | 26.31 | 0.46 ± 0.09 | 0.63 ± 0.15 | 0.76 ± 0.11 | 0.59 ± 0.17 | 0.64 ± 0.18 |
| Docosanoic acid | 28.00 | — | 0.01 ± 0.01 | — | 0.01 ± 0.01 | — |
| Docosanoic acid, ethyl ester | 28.21 | — | 0.45 ± 0.12 | — | 0.21 ± 0.05 | 0.23 ± 0.12 |
|
| ||||||
| Alcohols | ||||||
| Hexadecanol | 21.02 | 0.23 ± 0.05 | — | 0.19 ± 0.07 | 0.16 ± 0.04 | 0.16 ± 0.05 |
| Octadecanol | 23.87 | 0.26 ± 0.05 | 0.69 ± 0.16 | 0.19 ± 0.06 | 0.29 ± 0.08 | — |
| Eicosanol | 25.67 | 0.17 ± 0.03 | 0.55 ± 0.13 | 0.28 ± 0.08 | 0.21 ± 0.05 | 0.81 ± 0.28 |
| Docosanol | 27.33 | 0.23 ± 0.05 | 0.52 ± 0.15 | 0.23 ± 0.04 | 0.23 ± 0.04 | 0.73 ± 0.26 |
| Tetracosanol | 29.80 | 0.03 ± 0.01 | 0.07 ± 0.01 | 0.02 ± 0.01 | 0.07 ± 0.02 | 0.01 ± 0.01 |
|
| ||||||
| Waxy esters | ||||||
| Unidentified waxy ester 1 | 29.45 | 0.28 ± 0.08 | 0.98 ± 0.20 | — | 1.37 ± 0.47 | 0.75 ± 0.23 |
| Unidentified waxy ester 2 | 35.57 | 0.58 ± 0.10 | 2.61 ± 0.60 | 0.42 ± 0.08 | 2.84 ± 0.45 | 0.69 ± 0.26 |
| Unidentified waxy ester 3 | 38.06 | 0.23 ± 0.06 | 0.29 ± 0.06 | 0.20 ± 0.05 | 0.09 ± 0.03 | 0.37 ± 0.23 |
| Unidentified waxy ester 4 | 38.27 | 0.63 ± 0.11 | 1.78 ± 0.26 | 0.47 ± 0.10 | 2.26 ± 0.30 | 0.82 ± 0.16 |
|
| ||||||
| Others | ||||||
| Tetradecanone | 22.11 | 0.20 ± 0.05 | 0.27 ± 0.11 | 0.13 ± 0.03 | 0.15 ± 0.03 | 0.18 ± 0.06 |
| Unidentified Furanone | 24.19 | 0.12 ± 0.02 | 0.10 ± 0.03 | 0.06 ± 0.01 | — | — |
| Squalene | 30.07 | 0.93 ± 0.26 | 0.70 ± 0.10 | 0.66 ± 0.20 | 0.35 ± 0.04 | 0.40 ± 0.19 |
| Unidentified terpenoid 1 | 30.83 | 0.09 ± 0.03 | 0.07 ± 0.03 | 0.08 ± 0.02 | 0.03 ± 0.01 | 0.13 ± 0.07 |
| Unidentified terpenoid 2 | 31.94 | 0.48 ± 0.09 | — | 0.48 ± 0.12 | 0.05 ± 0.01 | — |