Figure 3.

The spleen contains proliferating myeloid cell progenitors that give rise to their progeny in vivo. A. CFU-GM shows colony formation in spleens of wt and apoE^−/− HCD mice (means ± SEM, n = 4). *P< 0.05. B. Phenotypic analysis of granulocyte and macrophage progenitors (GMP) in spleens of wt and apoE^−/− HCD mice. C, Enumeration of GMP in spleens and bone marrow of apoE^−/− mice fed HCD for up to 30 weeks. Linear regression was performed. D. Adoptive transfer of GFP⁺ GMP to wt and apoE^−/− HCD mice. Data show GFP cells in spleens 5 days after transfer. E. Enumeration of data above. Data show the fold increase from wt to apoE^−/− HCD mice of adoptively transferred GFP⁺ cells in the bone marrow (BM) and spleen (data shown are pooled from two independent experiments). F. CD45.1⁺ spleens from apoE^−/− HCD mice were transplanted to asplenic CD45.2⁺ apoE^−/− HCD mice for 10 days. Data show chimerism of GMP in spleens 10 days after transplantation. G. Cell cycle analysis of CD45.1⁺and CD45.2⁺ GMP in transplanted spleens shown in G. Numbers indicate percentage of cells in S/G₂/M phase (means ± SEM, n = 4). H. Monocyte accumulation in aortic tissue of the mice described in F and G. Representative of 2 independent experiments are shown.