Figure 1.

Morphology of the mitochondria in retinal microvasculature by TEM. (a) Sections prepared from the area near the retinal microvasculature were stained with uranyl acetate and lead citrate and viewed by TEM. Eight or more images/sections were recorded at 20,000× magnification. The photomicrograph is representative of three to four rat retinas per group; arrows: endothelial cell mitochondria. BM, basement membrane. Average mitochondrial (b) area, and (c) mitochondrial density in retinal endothelial cells were analyzed using Image J software (National Institutes of Health, Bethesda, MD; http://rsb.info.nih.gov/ij/index.html). The values are represented as the mean ± SD of 20 or more mitochondria per group. *P < 0.05 compared to values obtained from normal rats.