Table 1.
Primers and fluorescence-labeled probes of DENV and noncompetitive IC (MS2 RNA).
| Primer or probe | Sequence (5′–3′) | 5′ reporter | 3′ quencher |
|---|---|---|---|
| DENV-F (10546)-generic | GGTTAGAGGAGACCCCTCCC | ||
| DENV-R (10711)-generic | GAGACAGCAGGATCTCTGGTCT | ||
| MS2-F | CATAAGTTAGATGGCCGTCTGT | ||
| MS2-R | TAGAGACGACAACCATGCCAAAC | ||
| DENV-generic-MGB probe | AAACAGCATATTGACGCTGGGA | FAM | BHQ1 |
| MS2 probe | TCCAGACAACGTGCAACATATCGCGACGTATCGTGATATGG | HEX | BHQ1 |
| DENV-1-MGB probe | AACACCATGGGAAGCTGTACCCTG | FAM | BHQ1 |
| DENV-2-MGB probe | GAGATGAAGCTGTAGTCTCACTGG | HEX | BHQ1 |
| DENV-3-MGB probe | TGAGGGAAGCTGTACCTCCTTGCA | Texas Red | BHQ2 |
| DENV-4-MGB probe | CCCGAAGCCAGGAGGAAGCTGTACTCC | CY5 | BHQ2 |
Nucleotide numbering for generic primers DENV-F and -R is relative to DENV-1 (reference strain AF226685).
F: forward primer; R: reverse primer; MGB: minor groove binding; BHQ1 or 2: black hole quencher 1 or 2.