Table 3.

Dynamic range of generic real-time PCR^a.

DENV in vitro RNA copies in extraction	DENV in vitro RNA copies in RT	DENV in vitro RNA copies in PCRb	IC copies in PCRb	Number positives DENV in vitro RNA (%; mean Cq-value: SD)	Number positives IC (%; mean Cq-value: S.D)
1.25 × 107	107	106	500	12/12 (100%; 26.59; 0.54)	12/12 (100%; 35.82; 1.08)
1.25 × 106	106	105	500	12/12 (100%; 30.59; 0.46)	12/12 (100%; 32.67; 0.79)
1.25 × 105	105	104	500	12/12 (100%; 34.35; 1.09)	12/12 (100%; 32.97; 0.74)
1.25 × 104	104	103	500	8/12 (66.7%; 37.22; 0.73)	12/12 (100%; 33.73; 0.63)
1.25 × 103	103	102	500	1/12 (8%; 40.71)	12/12 (100%; 32.24; 0.60)
0	0	0	0	0/12	0/12

^aThe dynamic range of the generic PCR was determined on 10-fold dilution series of DENV in vitro RNA in the presence of a fixed amount of IC spiked into lysis buffer containing DENV negative human serum as background and for 12 replicates per dilution.

^bNumber of copies cDNA/PCR has been calculated as 1/12.5 of the extracted RNA. assuming 100% efficiency in extraction, reverse transcription (RT), and PCR.

SD: standard deviation.